Advances in Immunoassay Technology by N. Chiu, et. al.

By N. Chiu, et. al.

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2001). Selection and characterisation of recombinant single-chain antibodies to the hapten Aflatoxin-B1 from naive recombinant antibody libraries. J Immunol Methods, 254(12), 169-181. Ness, J. , Cherry, J. , Borchert, T. , Stemmer, W. , & Minshull, J. (1999). DNA shuffling of subgenomic sequences of subtilisin. Nat Biotechnol, 17(9), 893-896. Ohage, E. , & Steipe, B. (1999). Intrabody construction and expression. II. A synthetic catalytic Fv fragment. J Mol Biol, 291(5), 1129-1134. Orcutt, K. , Slusarczyk, A.

Hoet, R. M. (2005). Oligonucleotide-assisted cleavage and ligation: a novel directional DNA cloning technology to capture cDNAs. Application in the construction of a human immune antibody phage-display library. Nucleic Acids Res, 33(9), e81. , & Kane, M. (2008). Development of a high-affinity antidomoic acid sheep scFv and its use in detection of the toxin in shellfish. Anal Chem, 80(9), 3205-3212. , & Hall, J. C. (2001). Immunoaffinity purification of chlorimuron-ethyl from soil extracts prior to quantitation by enzyme-linked immunosorbent assay.

Modified fibers also showed lesser extent of non-specific binding caused due to physical adsorption and were more specific towards the detection of human blood. Fig. 13. 5% glutaraldehyde 4. Conclusions and prospects In our work pendent nitrile groups of multifilamentous polyacrylonitrile (PAN) fibers were reduced to amino groups using lithium aluminum hydride for different time of reduction and amine content was estimated by performing acid-base titrations. Modified fibers were characterized by spectroscopic and analytical techniques for the generation of amino groups.

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